Anisotropic Local Motions and Location of Amide Protons in Proteins

• Published in: Journal of the American Chemical Society, vol. 127, num. 14, p. 5180-5185
• Publication date: 2005

A new method has been developed to obtain dynamic and structural information about peptide planes in proteins by a combination of measurements of weak short-range cross-correlation rates R(HNN/NC') that are due to concerted fluctuations of the HN-N and N-C' dipole-dipole interactions and stronger long-range cross-correlation rates R(C'HN/HNN) and R(NHN/HNCa). The rates were interpreted using the axially sym. Gaussian axial fluctuation model (GAF). The oscillation amplitudes as well as the positions of HN atoms with respect to peptide planes in ubiquitin were detd. Most N-HN bonds were found not to lie exactly along the bisector of the N-C' and N-Ca bonds but to be slightly tilted toward the carbon-terminal side of the peptide. [on SciFinder (R)]

Reference

• LRMB-ARTICLE-2005-001
• doi:10.1021/ja043575v
• View record in Web of Science

Record created on 2006-02-22, modified on 2017-05-12