Hydrogen bonds of nucleic acids and local motions of proteins studied by nuclear spin relaxation

In my thesis I shall focus on the development of new NMR experimental methods to study structure and internal motions of two kinds of biological polymers, viz. proteins and nucleic acids. I shall investigate the anisotropic local motions of peptide planes and locate amide protons in proteins by measuring three dipole-dipole cross-correlated relaxation rate constants of single-quantum coherences involving the peptide backbone nuclei. I shall also estimate the internucleotide hydrogen bond lengths in nucleic acids from the measurement of the longitudinal auto-relaxation rate constants of both donor and acceptor nitrogen-15 nuclei of Watson-Crick base pairs. Finally I shall measure and discuss the temperature dependence of internucleotide nitrogen-nitrogen scalar couplings in nucleic acids.


Advisor(s):
Bodenhausen, Geoffrey
Year:
2004
Publisher:
Lausanne, EPFL
Other identifiers:
urn: urn:nbn:ch:bel-epfl-thesis3139-9


Note: The status of this file is: EPFL only


 Record created 2005-03-16, last modified 2018-01-27

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