Beyond fluorescence: advances in computational label- free full specificity in 3D quantitative phase microscopy
Despite remarkable progresses in quantitative phase imaging (QPI) microscopes, their wide acceptance is limited due to the lack of specificity compared with the well-established fluorescence microscopy. In fact, the absence of fluorescent tag prevents to identify subcellular structures in single cells, making challenging the interpretation of label-free 2D and 3D phase-contrast data. Great effort has been made by many groups worldwide to address and overcome such limitation. Different computational methods have been proposed and many more are currently under investigation to achieve labelfree microscopic imaging at single-cell level to recognize and quantify different subcellular compartments. This route promises to bridge the gap between QPI and FM for real-world applications.
WOS:001145124000001
2023-12-23
85
103054
REVIEWED
Funder | Grant Number |
Italian Ministry of University Research | |
Project POR CIRO (Campania Imaging for Research in Oncology) - Regione Campania (Italy) | |