Label-free biosensors, combined with miniaturized micro-electromechanical sensory platforms, offer an attractive solution for real-time and facile monitoring of biomolecules due to their high sensitivity and selectivity without the need for specifically labeling. Resonators have been acknowledged as an efficient technology for measuring biomolecular binding events including those involving nucleic acid and antibody. Among these, capacitive micromachined ultrasonic transducers (CMUTs) have emerged as a promising candidate for biosensing. However, their usage is often limited by the requirement for high voltage supply and continuous frequency tracking, which can result in significant parasitic effects and measurement errors. In this brief, we present a novel front-end interface circuit for a CMUTs-based biosensor. The circuit, fabricated using TSMC 0.18-mu m BipolarCMOS-DMOS (BCD) technology, incorporates an on-chip high voltage charge pump and feedback frequency monitoring. The CMUTs array features 20 x 20 circular cells, fabricated using a low-temperature direct bonding technology, with an experimental parallel-resonant frequency of 1.724 MHz and a high quality factor of up to 40.9. To fit the measured electrical characteristics, a five-element equivalent lumped element model is proposed. The high voltage charge pump provides an output voltage of similar to 20 V, while the feedback oscillator has a ms-level start-up time and a total power dissipation of 3.8 mW. The proposed frontend interface is designed to function as a stand-alone chip for CMUTs-based resonant biodetection.