The present disclosure relates to a microfluidic device comprising: a substrate; a culture chamber (130); a loading channel (170) in fluid communication with the culture chamber; at least one auxiliar channel (170-1, 5 170-2) extending from and in fluid communication with the loading channel, wherein the at least one auxiliary channel is so dimensioned such that a hydraulic resistance in the at least one auxiliary channel is higher than a hydraulic resistance in the loading channel; a test area (150) defined along the loading channel at a position between the loading channel and the at least one auxiliary channel; a first medium reservoir (110) in fluid communication with a first side of the test area; and a second medium reservoir (120) in fluid communication with a second side of the test area, the second side being different from the first side.