Repository logo

Infoscience

  • English
  • French
Log In
Logo EPFL, École polytechnique fédérale de Lausanne

Infoscience

  • English
  • French
Log In
  1. Home
  2. Academic and Research Output
  3. Journal articles
  4. Structure and regulation of the myotonic dystrophy kinase-related Cdc42-binding kinase
 
research article

Structure and regulation of the myotonic dystrophy kinase-related Cdc42-binding kinase

Truebestein, Linda
•
Antonioli, Sumire
•
Waltenberger, Elisabeth
Show more
April 6, 2023
Structure

Protein kinases of the dystonia myotonica protein kinase (DMPK) family are critical regulators of actomyosin contractility in cells. The DMPK kinase MRCK1 is required for the activation of myosin, leading to the develop-ment of cortical tension, apical constriction, and early gastrulation. Here, we present the structure, conforma-tion, and membrane-binding properties of Caenorhabditis elegans MRCK1. MRCK1 forms a homodimer with N-terminal kinase domains, a parallel coiled coil of 55 nm, and a C-terminal tripartite module of C1, pleckstrin homology (PH), and citron homology (CNH) domains. We report the high-resolution structure of the membrane -binding C1-PH-CNH module of MRCK1 and, using high-throughput and conventional liposome-binding assays, determine its binding to specific phospholipids. We further characterize the interaction of the C -termi-nal CRIB motif with Cdc42. The length of the coiled-coil domain of DMPK kinases is remarkably conserved over millions of years of evolution, suggesting that they may function as molecular rulers to position kinase activity at a fixed distance from the membrane.

  • Details
  • Metrics
Type
research article
DOI
10.1016/j.str.2023.02.002
Web of Science ID

WOS:000982532300001

Author(s)
Truebestein, Linda
Antonioli, Sumire
Waltenberger, Elisabeth
Gehin, Charlotte  
Gavin, Anne -Claude
Leonard, Thomas A.
Date Issued

2023-04-06

Publisher

CELL PRESS

Published in
Structure
Volume

31

Issue

4

Subjects

Biochemistry & Molecular Biology

•

Biophysics

•

Cell Biology

•

rho-associated kinase

•

myosin-binding subunit

•

protein-kinase

•

apical constriction

•

smooth-muscle

•

ctg repeat

•

c.-elegans

•

domain

•

mrck

•

phosphorylation

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
UPDANGELO  
Available on Infoscience
May 22, 2023
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/197778
Logo EPFL, École polytechnique fédérale de Lausanne
  • Contact
  • infoscience@epfl.ch

  • Follow us on Facebook
  • Follow us on Instagram
  • Follow us on LinkedIn
  • Follow us on X
  • Follow us on Youtube
AccessibilityLegal noticePrivacy policyCookie settingsEnd User AgreementGet helpFeedback

Infoscience is a service managed and provided by the Library and IT Services of EPFL. © EPFL, tous droits réservés