AC loss in the Nb3Sn cable-in-conduit conductors (CICC) usually decreases after the conductor undergoes electromagnetic cycling. This can be attributed to the increase of inter-strand resistance due to the detachment of the strand-bonding during cyclic loading. In the years 2018 to 2020, ITER launched a series of test campaigns in SULTAN test facility, whose aim was to study the T-cs degradation of TF conductors of all manufactures. Along the main scope of the project, an accompanying study of the AC loss evolution has been measured on two samples. The AC loss was measured prior to any electromagnetic loading, after 1, 5, 50 and 1000 cycles, and at the very end also after a thermal cycle to room temperature. Sinusoidal AC loss was measured in the frequency range of 0.1 to 1.0 Hz. The measured AC evolution will help to predict heat load generated in the TF coils during the initial phase of ITER operation. It may also serve as an input to analysts for deducing the evolution of inter-strand resistance during electromagnetic cycling. The main observation is that already after the first electromagnetic cycle the AC loss reduction is significant, namely 80-90% lower compared to the initial conductor state.