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research article
Environmentally Sensitive Color-Shifting Fluorophores for Bioimaging
September 28, 2020
We introduce color-shifting fluorophores that reversibly switch between a green and red fluorescent form through intramolecular spirocyclization. The equilibrium of the spirocyclization is environmentally sensitive and can be directly measured by determining the ratio of red to green fluorescence, thereby enabling the generation of ratiometric fluorescent probes and biosensors. Specifically, we developed a ratiometric biosensor for imaging calcium ions (Ca2+) in living cells, ratiometric probes for different proteins, and a bioassay for the quantification of nicotinamide adenine dinucleotide phosphate.
Type
research article
Web of Science ID
WOS:000573152700001
Authors
Publication date
2020-09-28
Publisher
Published in
Volume
59
Issue
49
Start page
21880
End page
21884
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
June 19, 2021
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