In the Mekong delta, inland-based shrimp breeding requires significant inflow of high-quality freshwater. In turn, discharge of substantial loads of poor-quality effluents negatively impacts adjacent water bodies and favors disease outbreaks. This project describes the implementation of a laboratory-based continuous closed recirculation aquaculture system composed of a constructed wetland (CW) with horizontal subsurface flow as a water treatment filter for mesohaline conditions, functioning under high loading rate (HLR = 1.54 m/d with HRT = 1.31 h). This CW was equipped of successive compartment dedicated to the successive elimination of the contaminants of interests. CW performance was measured over a complete growth cycle of the White-leg shrimps (Litopenaeus vannamei). Results showed that the designed system was pertinent, improving water quality of the shrimp culture substantially. Complete removal of nitrite was attained, with a concomitant reduction of respectively 78% and 76% of nitrate and COD. Bacteria enumeration tests showed that Vibrio sp. cells were fully removed, and that a 3 Log reduction was reached in total aerobic bacteria.