The ratio of branching fractions of the decays -> pK(-)mu(+)mu(-),RpK-1}, is measured for the first time using proton-proton collision data corresponding to an integrated luminosity of 4.7 fb(-1) recorded with the LHCb experiment at center-of-mass energies of 7, 8 and 13 TeV. In the dilepton mass-squared range 0.1 < q(2)< 6.0 GeV2/c(4) and the pK(-) mass range m(pK(-)) < 2600 MeV/c(2), the ratio of branching fractions is measured to be RpK-1=1.17-0.16+0.18 +/- 0.0$$ {R}{pK}<^>{-1}={1.17}{-0.16}<^>{+0.18}\pm 0.07 $$\end{document}, where the first uncertainty is statistical and the second systematic. This is the first test of lepton universality with b baryons and the first observation of the decay -> pK(-)e(+)e(-).