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research article
Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolution
November 18, 2020
We demonstrate gas cluster ion beam scanning electron microscopy (SEM), in which wide-area ion milling is performed on a series of thick tissue sections. This three-dimensional electron microscopy technique acquires datasets with <10 nm isotropic resolution of each section, and these can then be stitched together to span the sectioned volume. Incorporating gas cluster ion beam SEM into existing single-beam and multibeam SEM workflows should be straightforward, increasing reliability while improving z resolution by a factor of three or more.
Type
research article
Web of Science ID
WOS:000508582900037
Authors
Hayworth, Kenneth J.
•
Peale, David
•
Januszewski, Michal
•
•
Lu, Zhiyuan
•
Xu, C. Shan
•
Hess, Harald F.
Publication date
2020-11-18
Published in
Volume
17
Issue
1
Start page
68
End page
71
Peer reviewed
REVIEWED
Available on Infoscience
March 3, 2020
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