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book part or chapter
Preparation of 2D Crystals of Membrane Proteins for High-Resolution Electron Crystallography Data Collection
January 1, 2010
Cryo-EM Part A Sample Preparation and Data Collection
Electron crystallography is a powerful technique for the structure determination of membrane proteins as well as soluble proteins. Sample preparation for 2D membrane protein crystals is a crucial step, as proteins have to be prepared for electron microscopy at close to native conditions. In this review, we discuss the factors of sample preparation that are key to elucidating the atomic structure of membrane proteins using electron crystallography.
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Type
book part or chapter
Authors
Abeyrathne, Priyanka D.
•
Chami, Mohamed
•
Pantelic, Radosav S.
•
Goldie, Kenneth N.
•
Publication date
2010-01-01
Publisher
Published in
Cryo-EM Part A Sample Preparation and Data Collection
ISBN of the book
978-0-12-374906-2
Start page
25
End page
43
Volume
481
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
February 13, 2020