book part or chapter
3D Reconstruction from 2D Crystal Image and Diffraction Data
January 1, 2010
Cryo-EM, Part B: 3-D Reconstruction
Electron crystallography of 2D protein crystals can determine the structure of membrane embedded proteins at high resolution. Images or electron diffraction patterns are recorded with the electron microscope of the frozen hydrated samples, and the 3D structure of the proteins is then determined by computer data processing. Here we introduce the image-processing algorithms for crystallographic Fourier space based methods using the Medical Research Council (MRC) programs, and illustrate the usage of the software packages 2dx, XDP, and IPLT.
Type
book part or chapter
Author(s)
Date Issued
2010-01-01
Publisher
Published in
Cryo-EM, Part B: 3-D Reconstruction
ISBN of the book
978-0-12-384991-5
Start page
101
End page
129
Volume
482
Editorial or Peer reviewed
REVIEWED
Written at
OTHER
EPFL units
Available on Infoscience
February 13, 2020
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