A device for sorting biological entities is disclosed. The device comprises a channel to canalize the biological entities and a selector having slots to accommodate said entities. Means for detecting and analysing an optical parameter of the biological entities is coupled with the selector. The selector can switch from a rest position to a sorting position, or at least two sorting positions, based on the detected and analysed optical parameter, so that biological entities can be sorted on the basis of their optical properties. The device is particularly intended for sorting egg cells, zygotes, embryos, or larvae of an insect, an amphibian or a fish such as Xenopus laevis, C. elegans or zebfrafish Danio rerio.