Strategies for increasing the throughput of super-resolution microscopies

Fluorescence microscopy methods have been developed to circumvent the diffraction limit by exploiting nonlinearities in the interactions between light and fluorophores. Initially, these methods were up to orders of magnitude slower than standard microscopies. In recent years, a wide array of technological advances have increased the throughput of super-resolution microscopies, through parallelization, smart scanning or data processing, and sample expansion. Here, we review re cent innovations for increased throughput, some applications that have benefitted from them, and how they could be improved in the future.


Published in:
Current Opinion In Chemical Biology, 51, 84-91
Year:
Aug 01 2019
Publisher:
Oxford, ELSEVIER SCI LTD
ISSN:
1367-5931
1879-0402
Keywords:




 Record created 2019-09-14, last modified 2019-09-17


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