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research article
A Modular Ligation Strategy for Asymmetric Bivalent Nucleosomes Trimethylated at K36 and K27
May 2, 2019
In nature, individual histones in the same nucleosome can carry identical (symmetric) or different (asymmetric) post-translational modification (PTM) patterns, increasing the combinatorial complexity. Embryonic stem cells exhibit bivalent nucleosomes, some of which are marked by an asymmetric arrangement of H3K36me3 (an activating PTM) and H3K27me3 (a repressive PTM). Here we describe a modular synthetic method to access such asymmetrically modified nucleosomes and show that H3K36me3 inhibits the activity of the methyltransferase PRC2 locally while still prolonging its chromatin binding time.
Type
research article
Web of Science ID
WOS:000471317500004
Authors
Publication date
2019-05-02
Publisher
Published in
Volume
20
Issue
9
Start page
1124
End page
1128
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
June 28, 2019
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