Transmission Microsphere-Assisted Dark-Field Microscopy

Microsphere-assisted microscopy allows the limit of the diffraction of light to overcome while being non-invasive, full-field, label-free, and easy-to-implement. However, the observation of translucent samples remains difficult using a classical bright-field illumination. In this work, a method is presented for the inspection of quasi-transparent sub-diffraction-limited structures by using dark-field illumination in the transmission mode. Glass-imprint features, having a size of 250 nm, as well as fixed mouse brain cells have been visualized using the dark-field microsphere-assisted technique. The possibility to observe feature sizes up to 100 nm has been demonstrated in air using a 25-mu m-diameter glass microsphere combined with an optical microscope, opening new possibilities for biological imaging.


Published in:
Physica Status Solidi-Rapid Research Letters, 13, 2, 1800445
Year:
Feb 01 2019
Publisher:
Weinheim, WILEY-V C H VERLAG GMBH
ISSN:
1862-6254
1862-6270
Keywords:




 Record created 2019-06-18, last modified 2019-06-25


Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)