A Simple, Robust, and Low-Cost Method To Produce the PURE Cell Free System

We demonstrate a simple, robust, and low-cost method for producing the PURE cell-free transcription translation system. Our OnePot PURE system achieved a protein synthesis yield of 156 peg/mL at a cost of 0.09 USD/ktL, leading to a 14-fold improvement in cost normalized protein synthesis yield over existing PURE systems. The one pot method makes the PURE system easy to generate and allows it to be readily optimized and modified.


Published in:
Acs Synthetic Biology, 8, 2, 455-462
Year:
Feb 01 2019
Publisher:
Washington, AMER CHEMICAL SOC
ISSN:
2161-5063
Keywords:
Laboratories:




 Record created 2019-03-06, last modified 2019-06-19


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