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research article
Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9
November 1, 2018
Anti-CRISPR proteins are powerful tools for CRISPR-Cas9 regulation; the ability to precisely modulate their activity could facilitate spatiotemporally confined genome perturbations and uncover fundamental aspects of CRISPR biology. We engineered optogenetic anti-CRISPR variants comprising hybrids of AcrIIA4, a potent Streptococcus pyogenes Cas9 inhibitor, and the LOV2 photosensor from Avena sativa. Coexpression of these proteins with CRISPR-Cas9 effectors enabled light-mediated genome and epigenome editing, and revealed rapid Cas9 genome targeting in human cells.
Type
research article
Web of Science ID
WOS:000448827300028
Authors
Bubeck, Felix
•
Hoffmann, Mareike D.
•
•
Aschenbrenner, Sabine
•
Bietz, Andreas
•
Waldhauer, Max C.
•
Boerner, Kathleen
•
Fakhiri, Julia
•
Schmelas, Carolin
•
Dietz, Laura
Publication date
2018-11-01
Publisher
Published in
Volume
15
Issue
11
Start page
924
End page
927
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
December 13, 2018
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