Semisynthetic sensor proteins enable metabolic assays at the point of care

Monitoring metabolites at the point of care could improve the diagnosis and management of numerous diseases. Yet for most metabolites, such assays are not available. We introduce semisynthetic, light-emitting sensor proteins for use in paper-based metabolic assays. The metabolite is oxidized by nicotinamide adenine dinucleotide phosphate, and the sensor changes color in the presence of the reduced cofactor, enabling metabolite quantification with the use of a digital camera. The approach makes any metabolite that can be oxidized by the cofactor a candidate for quantitative point-of-care assays, as shown for phenylalanine, glucose, and glutamate. Phenylalanine blood levels of phenylketonuria patients were analyzed at the point of care within minutes with only 0.5 microliters of blood. Results were within 15% of those obtained with standard testing methods.


Published in:
Science, 361, 6407, 1122-1125
Year:
Sep 14 2018
Publisher:
Washington, AMER ASSOC ADVANCEMENT SCIENCE
ISSN:
0036-8075
1095-9203
Keywords:
Laboratories:




 Record created 2018-12-13, last modified 2019-12-05


Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)