Multicolor single-particle reconstruction of protein complexes

Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and coaligns multiple proteins from 2D super-resolution fluorescence images. To demonstrate our method, we generated multicolor 3D reconstructions of several proteins within the human centriole, which revealed their relative locations, dimensions and orientations.


Published in:
Nature Methods, 15, 10, 777-+
Year:
Oct 01 2018
ISSN:
1548-7091
1548-7105
Keywords:
Laboratories:




 Record created 2018-12-13, last modified 2019-12-05


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