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research article
Multicolor single-particle reconstruction of protein complexes
October 1, 2018
Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and coaligns multiple proteins from 2D super-resolution fluorescence images. To demonstrate our method, we generated multicolor 3D reconstructions of several proteins within the human centriole, which revealed their relative locations, dimensions and orientations.
Type
research article
Web of Science ID
WOS:000448820100022
Authors
Publication date
2018-10-01
Published in
Volume
15
Issue
10
Start page
777
End page
780
Peer reviewed
REVIEWED
Available on Infoscience
December 13, 2018
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