Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase

The isothermal amplification of DNA in minimally buffered conditions allows to perform and monitor nucleic acid amplification with minimal technological and operative requirements. We show in this work how phi29 can operate multiple displacement amplification in minimally buffered conditions producing, as a readout, pH shifts attaining subunits of pH.


Publié dans:
Chemical Communications, 54, 17, 2158-2161
Année
Feb 02 2018
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 Notice créée le 2018-03-08, modifiée le 2019-05-07

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