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research article
Luciferases with tunable emission wavelengths
We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. These tunable luciferases are generated by inserting the small luciferase NanoLuc into the structures of the self-labeling proteins SNAP-tag or HaloTag7 near their active sites. Labeling of the tags with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Luciferases with tunable colors have applications as reporter genes, for the construction of biosensors and in bioimaging.
Type
research article
Web of Science ID
WOS:000414764600038
Authors
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Sabbadini, Marina
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Hill, Nicholas
Publication date
2017
Publisher
Published in
Volume
56
Issue
46
Start page
14556
End page
14560
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
September 25, 2017
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