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book part or chapter
Rational Design and Applications of Semisynthetic Modular Biosensors: SNIFITs and LUCIDs
Stein, Viktor
Biosensors are used in many fields to measure the concentration of analytes, both in a cellular context and in human samples for medical care. Here, we outline the design of two types of modular biosensors: SNAP-tag-based indicators with a Fluorescent Intramolecular Tether (SNIFITs) and LUCiferase-based Indicators of Drugs (LUCIDs). These semisynthetic biosensors quantitatively measure analyte concentrations in vitro and on cell surfaces by an intramolecular competitive mechanism. We provide an overview of how to design and apply SNIFITs and LUCIDs.
Type
book part or chapter
Authors
Editors
Stein, Viktor
Publication date
2017
Publisher
Published in
Synthetic Protein Switches
ISBN of the book
978-1-4939-6938-8
Publisher place
New York, NY
Start page
101
End page
117
Series title/Series vol.
Methods in Molecular Biology; 1596
EPFL units
Available on Infoscience
September 8, 2017
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