Glycosaminoglycan Analysis by Cryogenic Messenger-Tagging IR Spectroscopy Combined with IMS-MS

We combine ion mobility spectrometry with cryogenic, messenger-tagging, infrared spectroscopy and mass spectrometry to . identify different isomeric disaccharides of chondroitin sulfate (CS) and heparan sulfate (HS), which are representatives of two major subclasses of glycosaminoglycans. Our analysis shows that while CS and HS disaccharide isomers have similar drift times, they can be uniquely distinguished by their vibrational spectrum between,similar to 3200 and 3700 cm(-1) due to their different OH hydrogen-bonding patterns. We suggest that this combination of techniques is well suited to identify and characterize glycan isomers directly, which presents tremendous challenges for existing methods.


Published in:
Analytical Chemistry, 89, 14, 7601-7606
Year:
2017
Publisher:
Washington, American Chemical Society
ISSN:
0003-2700
Laboratories:




 Record created 2017-09-05, last modified 2018-09-13


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