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book part or chapter
Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins: Case Study of the Mouse 5-HT3 Receptor
Lacapere, Jean-Jacques
There is growing interest in the use of mammalian protein expression systems, and in the use of antibodyderived chaperones, for structural studies. Here, we describe protocols ranging from the production of recombinant membrane proteins in stable inducible cell lines to biophysical characterization of purified membrane proteins in complex with llama antibody domains. These protocols were used to solve the structure of the mouse 5-HT3 serotonin receptor but are of broad applicability for crystallization or cryoelectron microscopy projects.
Type
book part or chapter
Authors
Hassaïne, Ghérici
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Deluz, Cédric
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Tomizaki, Takashi
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Desmyter, Aline
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Moreau, Christophe
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Peclinovska, Lucie
Editors
Lacapere, Jean-Jacques
Publication date
2017
Publisher
Published in
Membrane Protein Structure and Function Characterization
ISBN of the book
978-1-4939-7149-7
Publisher place
New York, NY
Start page
139
End page
168
Series title/Series vol.
Methods in Molecular Biology; 1635
Available on Infoscience
August 14, 2017
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