Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins: Case Study of the Mouse 5-HT3 Receptor

There is growing interest in the use of mammalian protein expression systems, and in the use of antibodyderived chaperones, for structural studies. Here, we describe protocols ranging from the production of recombinant membrane proteins in stable inducible cell lines to biophysical characterization of purified membrane proteins in complex with llama antibody domains. These protocols were used to solve the structure of the mouse 5-HT3 serotonin receptor but are of broad applicability for crystallization or cryoelectron microscopy projects.


Editor(s):
Lacapere, Jean-Jacques
Published in:
Membrane Protein Structure and Function Characterization, 139-168
Year:
2017
Publisher:
New York, NY, Springer New York
ISBN:
978-1-4939-7149-7
Keywords:
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 Record created 2017-08-14, last modified 2018-03-18

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