Extraction of cell-free DNA from urine, using polylysine-coated silica particles

DNA analysis is used for a variety of purposes, including disease diagnosis and DNA profiling; this involves extracting DNA from living organisms. In this study, we prepared polycationic silica particles to extract DNA that has the negatively charged phosphate backbone from solution. The coated particles were prepared by mixing conventional silica gel particles and poly-Lys; these particles could efficiently extract 1.3 mu g of cell-free DNA from 50 mL of (male) urine. It is expected that these easily prepared particles (just a mixture of two commercially available chemicals) can be used as a noninvasive diagnostic tool for genetic disorders such as cancer, diabetes, and hypertension.


Published in:
Analytical And Bioanalytical Chemistry, 409, 16, 4021-4025
Year:
2017
Publisher:
Heidelberg, Springer Heidelberg
ISSN:
1618-2642
Keywords:
Laboratories:




 Record created 2017-07-10, last modified 2018-03-17


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