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research article

Microsphere-based super-resolution scanning optical microscope

Huszka, Gergely  
•
Yang, Hui  
•
Gijs, Martin A. M.  
2017
Optics Express

High-refractive index dielectric microspheres positioned within the field of view of a microscope objective in a dielectric medium can focus the light into a so-called photonic nanojet. A sample placed in such nanojet can be imaged by the objective with super-resolution, i.e. with a resolution beyond the classical diffraction limit. However, when imaging nanostructures on a substrate, the propagation distance of a light wave in the dielectric medium in between the substrate and the microsphere must be small enough to reveal the sample's nanometric features. Therefore, only the central part of an image obtained through a microsphere shows super-resolution details, which are typically similar to 100 nm using white light (peak at lambda = 600 nm). We have performed finite element simulations of the role of this critical distance in the super-resolution effect. Super-resolution imaging of a sample placed beneath the microsphere is only possible within a very restricted central area of -10 mu m(2), where the separation distance between the substrate and the microsphere surface is very small (-1 mu m). To generate super-resolution images over larger areas of the sample, we have fixed a microsphere on a frame attached to the microscope objective, which is automatically scanned over the sample in a step-by-step fashion. This generates a set of image tiles, which are subsequently stitched into a single super-resolution image (with resolution of lambda/4-lambda/5) of a sample area of up to -10(4) mu m(2). Scanning a standard optical microscope objective with microsphere therefore enables super-resolution microscopy over the complete field-of-view of the objective. (C) 2017 Optical Society of America

  • Details
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Type
research article
DOI
10.1364/Oe.25.015079
Web of Science ID

WOS:000404189800106

Author(s)
Huszka, Gergely  
Yang, Hui  
Gijs, Martin A. M.  
Date Issued

2017

Publisher

Optical Soc Amer

Published in
Optics Express
Volume

25

Issue

13

Start page

15079

End page

15092

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LMIS2  
Available on Infoscience
July 10, 2017
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/138951
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