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  4. Label-free identification of activated T lymphocytes through tridimensional microsensors on chip
 
research article

Label-free identification of activated T lymphocytes through tridimensional microsensors on chip

Rollo, Enrica  
•
Tenaglia, Enrico  
•
Genolet, Raphael
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2017
Biosensors & Bioelectronics

Label-free approaches to assess cell properties ideally suit the requirements of cell-based therapeutics, since they permit to characterize cells with minimal perturbation and manipulation, at the benefit of sample recovery and re-employment for treatment. For this reason, label-free techniques would find sensible application in adoptive T cell-based immunotherapy. In this work, we describe the label-free and single-cell detection of in vitro activated T lymphocytes in flow through an electrical impedance-based setup. We describe a novel platform featuring 3D free-standing microelectrodes presenting passive upstream and downstream extensions and integrated into microfluidic channels. We employ such device to measure the impedance change associated with T cell activation at electrical frequencies maximizing the difference between non-activated and activated T cells. Finally, we harness the impedance signature of unstimulated T cells to set a boundary separating activated and non-activated clones, so to characterize the selectivity and specificity of the system. In conclusion, the strategy here proposed highlights the possible employment of impedance to assess T cell activation in label-free.

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Type
research article
DOI
10.1016/j.bios.2017.02.047
Web of Science ID

WOS:000401201300025

Author(s)
Rollo, Enrica  
Tenaglia, Enrico  
Genolet, Raphael
Bianchi, Elena  
Harari, Alexandre
Coukos, George
Guiducci, Carlotta  
Date Issued

2017

Publisher

Elsevier

Published in
Biosensors & Bioelectronics
Volume

94

Start page

193

End page

199

Subjects

Microfluidic flow cytometry

•

T cells

•

Immunotherapy

•

3D electrodes

•

Electrochemical impedance

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Microelectrodes

•

Microfluidics

•

Lab-on-chip devices

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
CLSE  
Available on Infoscience
July 10, 2017
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/138885
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