Tyrosine kinase JAK1 is associated with the granulocyte-colony- stimulating factor receptor and both become tyrosine-phosphorylated after receptor activation
Granulocyte-colony-stimulating factor (G-CSF) stimulates the proliferation and differentiation of cells of the neutrophil lineage by interaction with a specific receptor. Early signal transduction events following G-CSF receptor activation were studied. We detected tyrosine phosphorylation of both the G- CSF receptor and the protein tyrosine kinase JAK1 following G-CSF binding to the human G-CSF receptor. In vitro, the kinase activity of JAK1 was increased by G-CSF stimulation. Coimmunoprecipitation of JAK1 with the G-CSF receptor suggested a physical association which existed prior to G-CSF stimulation.
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Nicholson, S. E., Oates, A. C., Harpur, A. G., Ziemiecki, A., Wilks, A. F., & Layton, J. E. (1994). Tyrosine kinase JAK1 is associated with the granulocyte-colony- stimulating factor receptor and both become tyrosine-phosphorylated after receptor activation. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 91(8), 2985–2988. https://doi.org/10.1073/pnas.91.8.2985
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2-s2.0-0028344499
1994
91
8
2985
2988
REVIEWED
OTHER