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research article
4D Cryo-Electron Microscopy of Proteins
Cryo-electron microscopy is a form of transmission electron microscopy that has been used to determine the 3D structure of biological specimens in the hydrated state and with high resolution. We report the development of 4D cryo-electron microscopy by integrating the fourth dimension, time, into this powerful technique. From time-resolved diffraction of amyloid fibrils in a thin layer of vitrified water at cryogenic temperatures, we were able to detect picometer movements of protein molecules on a nanosecond time scale. Potential future applications of 4D cryo-electron microscopy are numerous, and some are discussed here. © 2013 American Chemical Society.
Type
research article
Authors
Publication date
2013
Published in
Volume
135
Issue
51
Start page
19123
End page
19126
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
February 13, 2017
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