000225524 001__ 225524
000225524 005__ 20190317000632.0
000225524 037__ $$aPOST_TALK
000225524 245__ $$aOptoMEA: a new tool for combining local optical activation of compounds with distributed MEA recordings
000225524 269__ $$a2007
000225524 260__ $$c2007
000225524 336__ $$aPosters
000225524 520__ $$aSince their introduction, Micro-Electrode Arrays (MEAs) have been exploited as devices providing distributed information about learning, memory and information processing in a cultured neuronal network, thus changing the field of view from single cell level (glass pipettes) to the scale of the complex network. MEAs represent a growing technology for the study of the functional activity of neuronal networks providing the possibility to gain information about the spatio-temporal dynamics of the network and to allow recordings of electrical activity over periods of time not compatible with conventional electrodes at several sites in parallel. More recently, according to the trend aimed at the reduction of animal tests, MEAs have been exploited as in vitro biosensors to monitor both acute and chronic effects of drugs on neuronal networks in physiological or pathophysiological conditions. On the contrary, the presence of stimulus artefacts and the poorly controlled spread of electrical stimuli in the culture medium limit the applicability of MEAs for neuronal stimulation. Although the problem of artefacts has been recently solved using blanking circuits, the problem of spreading of electrical signals is inherent to the use of electrical stimulations in a conductive volume. Moreover, because neurons are naturally interconnected in complex networks electrical stimuli applied to a region of the network may activate neurons of that region and also fibres of passage coming from neurons of other regions. To overcome these limitations, in addition to electrophysiological techniques, optical methods for the stimulation of neurons have been used for relatively a long time, i.e. by caged compound activation. Here we present the new OptoMEA tool where local light stimulations were obtained switching caged glutamate in the active form by UV light pulses using optical fibres exactly aligned at the MEA electrodes. This tool allows us to activate the network or to deliver other active compounds in specific regions of the network and to monitor their effects on the overall network functioning. This methodology may turn out to be extremely useful for testing the ability of drugs to affect neuronal properties as well as alterations in inter- and intra-neuronal communication.
000225524 700__ $$0249241$$g254787$$aGhezzi, Diego
000225524 700__ $$aHeuschkel, Marc Olivier
000225524 700__ $$aMenegon, Andrea
000225524 700__ $$aPedrocchi, Alessandra
000225524 700__ $$aMantero, Sara
000225524 700__ $$aMakohliso, Solomzi
000225524 700__ $$aValtorta, Flavia
000225524 700__ $$aFerrigno, Giancarlo
000225524 7112_ $$dNovember 3-10, 2007$$cSan Diego, California, USA$$aNeuroscience 2007 (SFN)
000225524 773__ $$tAbstracts of the 37th Society for Neuroscience Annual Meeting
000225524 8564_ $$uhttps://infoscience.epfl.ch/record/225524/files/Proceedings%20of%20SFN%202007a.pdf$$zn/a$$s82973$$yn/a
000225524 909C0 $$xU13047$$0252540$$pLNE
000225524 909CO $$ooai:infoscience.tind.io:225524$$qGLOBAL_SET$$pSTI$$pposter
000225524 917Z8 $$x254787
000225524 937__ $$aEPFL-POSTER-225524
000225524 973__ $$aOTHER
000225524 980__ $$aPOSTER