Confocal microscopy via multimode fibers: fluorescence bandwidth

We recently described a method for confocal reflection imaging through fibers, as a way to increase contrast when imaging unstained biological specimens. Using a transmission matrix, focused spots can be created at the distal end of a fiber. The backscattered field coming back from the sample can be filtered using optical correlation to obtain spatial selectivity in the detection. In this proceedings article, we briefly review the working principle of this method, and we discuss how the scheme could be adapted to confocal fluorescence imaging. In particular, we show simulations of the achievable detection bandwidth when using step-index multimode fibers as imaging devices.


Editor(s):
Bifano, Tg
Kubby, J
Gigan, S
Published in:
Adaptive Optics And Wavefront Control For Biological Systems Ii, 9717, UNSP 97171C
Presented at:
Conference on Adaptive Optics and Wavefront Control for Biological Systems II, San Francisco, CA, FEB 13-15, 2016
Year:
2016
Publisher:
Bellingham, Spie-Int Soc Optical Engineering
ISSN:
0277-786X
ISBN:
978-1-62841-951-1
Keywords:
Laboratories:




 Record created 2017-01-24, last modified 2018-09-13


Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)