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research article

Silver nanoparticle-protein interactions in intact rainbow trout gill cells

Yue, Yang
•
Behra, Renata
•
Sigg, Laura
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2016
Environmental Science-Nano

Upon contact with biota, nanoparticles can bind to proteins, which coat the nanoparticles and form a nanoparticle-protein corona. Knowledge of corona proteins is therefore important for a mechanistic understanding of how nanoparticles interact with biomolecules in cells. Here we present the first study to reveal the identity of corona proteins from silver nanoparticle (AgNPs)-exposed living vertebrate cells. The cells are from a rainbow trout (Oncorhynchus mykiss) gill cell line, RTgill-W1, representing the interface between the aquatic environment and one of its model species. Subcellular fractionation allowed AgNP-protein corona complexes to be recovered from intact subcellular compartments and proteins lysed from the AgNPs to be detected by mass spectrometry. The identified proteins mark the trail of AgNPs processing in the cells like a forensic fingerprint: the cells take up the AgNPs via endocytic processes and store the particles in endosomal/lysosomal compartments. Moreover, stress response proteins were recovered in the AgNPs protein corona. In this way, we established a list of AgNPs susceptible proteins which can be investigated further in targeted nanoparticle-protein interaction. As a proof of principle, we demonstrate that Na+/K+-ATPase, identified from the corona and a known key protein in ion regulation in gill cells, is inhibited in its activity by AgNPs, confirming previously published in vivo experiments. The developed methodology is broadly applicable to other nanoparticles and cell types, representing a valuable tool for mechanistic nanoparticle-cell interaction studies, ranging from environmental and human risk assessment to biomedicine. In this way, our research also contributes to safer particle design.

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Type
research article
DOI
10.1039/c6en00119j
Web of Science ID

WOS:000385257900023

Author(s)
Yue, Yang
Behra, Renata
Sigg, Laura
Suter, Marc J. -F.
Pillai, Smitha
Schirmer, Kristin  
Date Issued

2016

Publisher

Royal Soc Chemistry

Published in
Environmental Science-Nano
Volume

3

Issue

5

Start page

1174

End page

1185

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
TOX  
Available on Infoscience
November 21, 2016
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/131306
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