With the rapid growth and increased consumption of biofuels worldwide, and the multitude of policy decisions supporting this expansion, growing concerns about the biofuels sustainability have arisen. Therefore, the European project "ITAKA", aiming at supporting the development of aviation biofuels in an economically, socially, and environmentally sustainable manner has devoted considerable effort to take sustainability into account, in a quantitative and qualitative manner. More precisely, a robust assessment of a lifecycle greenhouse gas (GHG) calculation for the produced bio jet fuel have been set up, using the RSB EU RED methodology. This pathway includes feedstock production, feedstock processing, biofuel production, biofuel distillation, and all transport steps involved. A significant reduction in GHG emissions (up to 66%) has been demonstrated.