Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension

Disposable orbitally shaken TubeSpin bioreactor 600 tubes (TS600s) were recently developed for the bench-scale cultivation of animal cells in suspension. Here we compared batch cultures of Sf9 insect cells in TS600s, spinner flasks, and shake flasks. Superior cell growth was observed in TS600s and shake flasks as compared with spinner flasks, and more favorable oxygen-enriched cell culture conditions were observed in TS600s as compared with either spinner or shake flasks. The results demonstrated the suitability of TS600s as a disposable vessel for the cultivation of Sf9 cells in suspension. (C) 2016 Elsevier Inc. All rights reserved.


Published in:
Analytical Biochemistry, 505, 26-28
Year:
2016
Publisher:
San Diego, Elsevier
ISSN:
0003-2697
Keywords:
Laboratories:




 Record created 2016-07-19, last modified 2018-03-17


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