Loading...
research article
Microsurgery-aided in-situ force probing reveals extensibility and viscoelastic properties of individual stress fibers
March 30, 2016
Actin-myosin filament bundles (stress fibers) are critical for tension generation and cell shape, but their mechanical properties are difficult to access. Here we propose a novel approach to probe individual peripheral stress fibers in living cells through a microsurgically generated opening in the cytoplasm. By applying large deformations with a soft cantilever we were able to fully characterize the mechanical response of the fibers and evaluate their tension, extensibility, elastic and viscous properties.
Loading...
Name
srep23722.pdf
Type
Publisher's version
Access type
openaccess
License Condition
CC BY
Size
1.69 MB
Format
Adobe PDF
Checksum (MD5)
a881596957bc14d782b611b335b1080d