Microsurgery-aided in-situ force probing reveals extensibility and viscoelastic properties of individual stress fibers

Actin-myosin filament bundles (stress fibers) are critical for tension generation and cell shape, but their mechanical properties are difficult to access. Here we propose a novel approach to probe individual peripheral stress fibers in living cells through a microsurgically generated opening in the cytoplasm. By applying large deformations with a soft cantilever we were able to fully characterize the mechanical response of the fibers and evaluate their tension, extensibility, elastic and viscous properties.


Published in:
Scientific Reports, 6, 23722
Year:
2016
Publisher:
London, Nature Publishing Group
ISSN:
2045-2322
Laboratories:




 Record created 2016-07-19, last modified 2018-03-17


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