000215955 001__ 215955
000215955 005__ 20190317000406.0
000215955 0247_ $$2doi$$a10.1039/C5SC04919A
000215955 022__ $$a2041-6520
000215955 02470 $$2ISI$$a000374859300003
000215955 037__ $$aARTICLE
000215955 245__ $$aSensitive and fast identification of bacteria in blood samples by immunoaffinity mass spectrometry for quick BSI diagnosis
000215955 269__ $$a2016
000215955 260__ $$bRoyal Soc Chemistry$$c2016$$aCambridge
000215955 300__ $$a9
000215955 336__ $$aJournal Articles
000215955 520__ $$aBloodstream infections rank among the most serious causes of morbidity and mortality in hospitalized patients, partly due to the long period (up to one week) required for clinical diagnosis. In this work, we have developed a sensitive method to quickly and accurately identify bacteria in human blood samples by combining optimized matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) and efficient immunoaffinity enrichment/separation. A library of bacteria reference mass spectra at different cell numbers was firstly built. Due to a reduced sample spot size, the reference spectra could be obtained from as few as 10 to 102 intact bacterial cells. Bacteria in human blood samples were then extracted using antibodies-modified magnetic beads for MS fingerprinting. By comparing the sample spectra with the reference spectra based on a cosine correlation, bacteria with concentrations as low as 500 cells per mL in blood serum and 8000 cells per mL in whole blood were identified. The proposed method was further applied to positive clinical blood cultures (BCs) provided by a local hospital, where Escherichia coli and Staphylococcus aureus were identified. Because of the method’s high sensitivity, the BC time required for diagnosis can be greatly reduced. As a proof of concept, whole blood spiked with a low initial concentration (102 or 103 cells per mL) of bacteria was cultured in commercial BC bottles and analysed by the developed method after different BC times. Bacteria were successfully identified after 4 hours of BC. Therefore, an entire diagnostic process could be accurately accomplished within half a day using the newly developed method, which could facilitate the timely determination of appropriate anti-bacterial therapy and decrease the risk of mortality from bloodstream infections.
000215955 700__ $$0248531$$g244161$$aZhu, Yingdi
000215955 700__ $$0242739$$g105258$$aQiao, Liang
000215955 700__ $$0240245$$g114390$$aPrudent, Michel
000215955 700__ $$0245816$$g217921$$aBondarenko, Alexandra
000215955 700__ $$0244747$$g207324$$aGasilova, Natalia
000215955 700__ $$aMöller, Siham Beggah
000215955 700__ $$0240309$$g146269$$aLion, Niels
000215955 700__ $$aPick, Horst
000215955 700__ $$aGong, Tianqi
000215955 700__ $$aChen, Zhuoxin
000215955 700__ $$aYang, Pengyuan
000215955 700__ $$aLovey, Lysiane Tissières
000215955 700__ $$0242739$$g105258$$aGirault, Hubert H.
000215955 773__ $$j7$$tChemical Science$$k5$$q2935-3452
000215955 8564_ $$uhttps://infoscience.epfl.ch/record/215955/files/c5sc04919a.pdf$$zPublisher's version$$s1474912$$yPublisher's version
000215955 909C0 $$xU10100$$0252090$$pLEPA
000215955 909CO $$ooai:infoscience.tind.io:215955$$qGLOBAL_SET$$pSB$$particle
000215955 917Z8 $$x208650
000215955 917Z8 $$x208650
000215955 917Z8 $$x208650
000215955 917Z8 $$x208650
000215955 937__ $$aEPFL-ARTICLE-215955
000215955 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000215955 980__ $$aARTICLE