Elucidating the role of Hmx1 during mouse development
This thesis focuses on the study of H6 homeobox 1 (HMX1) gene, a homeobox-containing transcription factor involved in sensory organ and eye development and responsible for the oculo-auricular syndrome of Schorderet-Munier-Franceschetti. The purpose of my study is to better understand the mechanism and role of this transcription factor. My thesis is divided into three parts. The first part proposes a predictive promoter model approach to identify new HMX1 target gene. The second part concerns an animal model of Hmx1 mutation, the dumbo mouse model, and brings a new insight into lethal craniofacial defects. The third part concerns the characterization of the visual capacity of the dumbo mice and a description of a new pattern of vision-linked defects. In the first part we propose a predictive promoter model approach based on transcriptomic analysis of post-natal day 15 mouse retina from dumbo and wild type mouse. This method revealed that Sgcg, Tshz2 and Slc6a9 were Hmx1 targets in the mouse retina. Ptpro, Sema3f, and Epha6 belong to the retinal axon guidance pathway and play an important role in retinotopic mapping. Significant enrichment of HMX1 binding site confirmed this pathway and we validated the transcriptional activity of Hmx1 on Ptpro, Sema3f and Sgcg by Luciferase assay. In the second part of the project we investigate the dumbo mouse model, carrying a stop codon in Hmx1, and representing the first animal model for the oculo-auricular syndrome of Schorderet-Munier-Franceschetti. The homozygous Hmx1dmbo is a semi-lethal mutation and stillborns present severe craniofacial defects, including cleft palate, chest defect and bone hypoplasia of maxilla, zygomatic process, mandible and frontal. In the third part of the thesis we provide a complete description of the vision in dumbo mouse. Despite a normal retinal phenotype, the vision of this mouse is completely impaired. We demonstrate that this was caused by abnormal retinotopic mapping and optic nerve hypoplasia. In dumbo mice the retinal ganglion cells axon projections failed to target the visual output, namely the dorsal lateral geniculate nucleus and the superior colliculus, and present abnormal patterning.
Programme doctoral Neurosciences
Faculté des sciences de la vie
Institut des neurosciences
Jury: Prof. Johannes Gräff (président) ; Prof. Daniel Francis Schorderet (directeur de thèse) ; Prof. Ralf Schneggenburger, Prof. Hélène Dollfus, Prof. Francis Munier (rapporteurs)
Public defense: 2015-10-29
Record created on 2015-10-28, modified on 2016-08-09