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research article
SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy
Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR–Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.
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articles-ncomms9497.pdf
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Publisher's version
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openaccess
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CC BY
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1.38 MB
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Adobe PDF
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