Labelling of fusion proteins with synthetic probes
The invention relates to new proteins called alkylcytosine transferases (ACTs) derived from O<6>-alkylguanine-DNA alkyltransferase, and to substrates for ACTs specifically transferring a label to these ACTs and to fusion proteins comprising these. The substrates according of the invention are substituted cytosines of formula (I) wherein R1 is an aromatic or a heteroaromatic group, or an optionally substituted unsaturated alkyl, cycloalkyl or heterocyclyl group with the double bond connected to OCH2- R2 is a linker and L is a label or a plurality of same or different labels. The invention further relates to methods of transferring label L from these substrates of formula (I) to ACTs and ACT fusion proteins. The system of ACT-compound of formula (I) is particularly suitable for double labelling studies together with the known system O6-alkylguanine-DNA alkyltransferase (AGT)-benzylguanines.
36954295
Alternative title(s) : (de) Markieren von fusionsproteinen mit synthetischen sonden (fr) Marquage de protéines de fusion avec des sondes synthétiques
TTO:6.0692
Patent number | Country code | Kind code | Date issued |
US8623627 | US | B2 | 2014-01-07 |
EP2049499 | EP | B1 | 2012-10-31 |
US2012237961 | US | A1 | 2012-09-20 |
US8227602 | US | B2 | 2012-07-24 |
US2011207157 | US | A1 | 2011-08-25 |
JP2009544660 | JP | A | 2009-12-17 |
CN101516857 | CN | A | 2009-08-26 |
EP2049499 | EP | A1 | 2009-04-22 |
WO2008012296 | WO | A1 | 2008-01-31 |
EP1882688 | EP | A1 | 2008-01-30 |