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There is provided a method for plasmid DNA extraction to reproducibly yield relatively crude preparation which is suitable for transfection into mammalian cells. The inventors have determined that the high level of purification previously thought to be necessary in order to successfully allow transfection of the plasmid DNA into cells is not required. The inventors have developed a method of producing a transfectable plasmid preparation that can be performed on a large scale that avoids the use of specialized equipment and reagents.
Type
patent
EPO Family ID
22890443
Inventors
Note
Alternative title(s) : (fr) Transfection a l'aide de preparations plasmidiques
TTO classification
TTO:6.0234
Patent number | Country code | Kind code | Date issued |
WO0226966 | WO | A3 | 2002-10-17 |
AU9014701 | AU | A | 2002-04-08 |
WO0226966 | WO | A2 | 2002-04-04 |
Available on Infoscience
September 22, 2015
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