Cumulant microscopy
The invention describes a method and a microscopy system for imaging and analysing stochastically and independently blinking point-like emitters. A multiple-order cumulants analysis in conjunction with an established blinking model enables the extraction of super-resolved environment-related parameter maps, such as molecular state lifetimes, concentration and brightness distributions of the emitter. In addition, such parameter maps can be used to compensate for the non-linear brightness and blinking response of higher-order cumulant images - used for example in Super-resolution Optical Fluctuation Imaging (SOFI) - to generate a balanced image contrast. Structures that otherwise would be masked by brighter regions in the conventional cumulant image become visible in the balanced cumulant image. The invention furthermore provides a method for the spectral unmixing of multi- colour samples using spectral cross-cumulants.
46208106
Alternative title(s) : (de) Kumulantenmikroskopie (fr) Microscopie des cumulants
TTO:6.1091
DOI | Country code | Kind code | Date issued |
EP2721441 | EP | B1 | 2019-09-18 |
US9658442 | US | B2 | 2017-05-23 |
US2014198198 | US | A1 | 2014-07-17 |
EP2721441 | EP | A1 | 2014-04-23 |
WO2012172440 | WO | A1 | 2012-12-20 |
EP2535755 | EP | A1 | 2012-12-19 |