Local Amplitude and Phase Retrieval Method for Digital Holography Applied to Microscopy

We present a numerical two-step reconstruction procedure for digital off-axis Fresnel holograms. First, we retrieve the amplitude and phase of the object wave in the CCD plane. For each point we solve a weighted linear set of equations in the least-squares sense. The algorithm has O(N) complexity and gives great flexibility. Second, we numerically propagate the obtained wave to achieve proper focus. We apply the method to microscopy and demonstrate its suitability for the real time imaging of biological samples.


Published in:
Proceedings of the SPIE European Conference on Biomedical Optics: Novel Optical Instrumentation for Biomedical Applications (ECBO'03), 5143, Münich, Federal Republic of Germany, 210–214
Year:
2003
Publisher:
SPIE
Laboratories:




 Record created 2015-09-18, last modified 2018-03-17

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