Characterization of intracellular metabolic states in the origin of the metabolic reprogramming of cancer cells
Cancer cells have been observed to undergo a metabolic reprogramming in which glycolytic fluxes are upregulated whereas oxidative phosphorylation is downregulated. This metabolic phenotype is known as the Warburg effect and it is observed even under high oxygen conditions. To study the mechanism behind this metabolic reprogramming: - We build a reduced model (RedHuman) from the GEM Recon 2 that is representative of the central carbon metabolism of mammalian cells; - We combine fluxomics and metabolomics data to simulate a normal mammalian cells metabolic phenotype during exponential growth phase and the metabolic phenotype of a cancer cell; - We then analyze the feasible flux ranges of the two simulated metabolic phenotypes.
Record created on 2015-09-04, modified on 2016-08-09