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  4. Venus trap in the mouse embryo reveals distinct molecular dynamics underlying specification of first embryonic lineages
 
research article

Venus trap in the mouse embryo reveals distinct molecular dynamics underlying specification of first embryonic lineages

Dietrich, Jens-Erik
•
Panavaite, Laura
•
Gunther, Stefan
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2015
EMBO reports

Mammalian development begins with the segregation of embryonic and extra-embryonic lineages in the blastocyst. Recent studies revealed cell-to-cell gene expression heterogeneity and dynamic cell rearrangements during mouse blastocyst formation. Thus, mechanistic understanding of lineage specification requires quantitative description of gene expression dynamics at a single-cell resolution in living embryos. However, only a few fluorescent gene expression reporter mice are available and quantitative live image analysis is limited so far. Here, we carried out a fluorescence gene-trap screen and established reporter mice expressing Venus specifically in the first lineages. Lineage tracking, quantitative gene expression and cell position analyses allowed us to build a comprehensive lineage map of mouse pre-implantation development. Our systematic analysis revealed that, contrary to the available models, the timing and mechanism of lineage specification may be distinct between the trophectoderm and the inner cell mass. While expression of our trophectoderm-specific lineage marker is upregulated in outside cells upon asymmetric divisions at 8- and 16-cell stages, the inside-specific upregulation of the inner-cell-mass marker only becomes evident at the 64-cell stage. This study thus provides a framework toward systems-level understanding of embryogenesis marked by high dynamicity and stochastic variability.

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Type
research article
DOI
10.15252/embr.201540162
Web of Science ID

WOS:000358952500019

PubMed ID

26142281

Author(s)
Dietrich, Jens-Erik
Panavaite, Laura
Gunther, Stefan
Wennekamp, Sebastian
Groner, Anna C
Pigge, Anton
Salvenmoser, Stefanie
Trono, Didier  
Hufnagel, Lars
Hiiragi, Takashi
Date Issued

2015

Published in
EMBO reports
Volume

16

Issue

8

Start page

1005

End page

1021

Subjects

gene trap

•

lineage map

•

live imaging

•

mouse

•

pre-implantation development

Editorial or Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
LVG  
Available on Infoscience
July 16, 2015
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/116333
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