Fluorescent Labeling of SNAP-Tagged Proteins in Cells

One of the most prominent self-labeling tags is SNAP-tag. It is an in vitro evolution product of the human DNA repair protein O 6-alkylguanine-DNA alkyltransferase (hAGT) that reacts specifically with benzylguanine (BG) and benzylchloropyrimidine (CP) derivatives, leading to covalent labeling of SNAP-tag with a synthetic probe (Gronemeyer et al., Protein Eng Des Sel 19:309–316, 2006; Curr Opin Biotechnol 16:453–458, 2005; Keppler et al., Nat Biotechnol 21:86–89, 2003; Proc Natl Acad Sci U S A 101:9955–9959, 2004). SNAP-tag is well suited for the analysis and quantification of fused target protein using fluorescence microscopy techniques. It provides a simple, robust, and versatile approach to the imaging of fusion proteins under a wide range of experimental conditions.


Editor(s):
Gautier, Arnaud
Hinner, Marlon J.
Published in:
Site-Specific Protein Labeling, 107-118
Year:
2014
Publisher:
New York, NY, Springer New York
ISBN:
978-1-4939-2271-0
Laboratories:




 Record created 2015-06-23, last modified 2018-03-17


Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)