An automated microfluidic platform for long-term high-resolution imaging of C. elegans

We describe a microfluidic platform for the automated culture, treatment and long-term highresolution imaging of Caenorhabditis elegans nematodes under normal physiological conditions. Our device features: (i) a microfluidic design tailored for the isolation of L4 larvae from a mixed larval population and for successive culture and treatment; (ii) a worm immobilization method, based on the thermo-reversible gelation of a biocompatible polymer inside the microfluidic chip, thereby enabling high-resolution imaging; (iii) an integrated temperature control system, both to ensure viable environmental conditions for C. elegans culture and to steer the worm immobilization/release process.


Published in:
Proceed. MicroTAS 2014, 709-711
Presented at:
The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014), San Antonio, TX, USA, 26-30 October
Year:
2014
Keywords:
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 Record created 2015-04-20, last modified 2018-03-18

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